Review



px552 u6 sp grna cmv gfp  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Addgene inc px552 u6 sp grna cmv gfp
    Px552 U6 Sp Grna Cmv Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px552+cmv+gfp+plasmid/PX552+(Plasmid+%2360958)/10__1134_slash_s0006297921070051-49-27-40
    Average 95 stars, based on 104 article reviews
    px552 u6 sp grna cmv gfp - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Utility of Self-Destructing CRISPR/Cas Constructs for Targeted Gene Editing in the Retina.
    Article Snippet: AgeI (catalog no. R0552S; New England Biolabs) digested pX551 plasmid was used as a positive control. .. Four SpCas9 sgRNAs were then cloned into a pX552‐CMV‐GFP plasmid (modified from Addgene #60958, by replacing the hSYN1 promoter with a CMV promoter) at the SapI restriction site for in vitro validation. .. Subsequently, the selected SpCas9 sgRNA (SpCas9 sgRNA4) was sub‐cloned into an AAV package plasmid (pX552‐hSYN1‐mCherry‐YFP sgRNA2, sgRNA6 or pX552‐LacZ sgRNA) at the MluI (catalog no. R3198; New England Biolabs) restriction site to generate YFP or LacZ targeting kamikaze CRISPR/Cas9 construct.

    Article Title: Efficacy and dynamics of self-targeting CRISPR/Cas constructs for gene editing in the retina
    Article Snippet: Agel (catalog no. R0552S; New England Biolabs) digested pX551 plasmid was used as a positive control. .. Four SpCas9 sgRNAs were then cloned into a pX552-CMV-GFP plasmid (modified from Addgene #60958, by replacing the hSyn promoter with a CMV promoter) at the SapI restriction site for in vitro validation. .. Subsequently, the selected SpCas9 sgRNA (SpCas9 sgRNA4) was sub-cloned into a AAV package plasmid (pX552-hsyn-mCherry-YFP sgRNA2, sgRNA6 or pX552-LacZ sgRNA) at the MluI (catalog no. R3198; New England Biolabs) restriction site to generate YFP or LacZ targeting kamikaze CRISPR/Cas construct.

    Plasmid Preparation:

    Article Title: Utility of Self-Destructing CRISPR/Cas Constructs for Targeted Gene Editing in the Retina.
    Article Snippet: AgeI (catalog no. R0552S; New England Biolabs) digested pX551 plasmid was used as a positive control. .. Four SpCas9 sgRNAs were then cloned into a pX552‐CMV‐GFP plasmid (modified from Addgene #60958, by replacing the hSYN1 promoter with a CMV promoter) at the SapI restriction site for in vitro validation. .. Subsequently, the selected SpCas9 sgRNA (SpCas9 sgRNA4) was sub‐cloned into an AAV package plasmid (pX552‐hSYN1‐mCherry‐YFP sgRNA2, sgRNA6 or pX552‐LacZ sgRNA) at the MluI (catalog no. R3198; New England Biolabs) restriction site to generate YFP or LacZ targeting kamikaze CRISPR/Cas9 construct.

    Article Title: Efficacy and dynamics of self-targeting CRISPR/Cas constructs for gene editing in the retina
    Article Snippet: Agel (catalog no. R0552S; New England Biolabs) digested pX551 plasmid was used as a positive control. .. Four SpCas9 sgRNAs were then cloned into a pX552-CMV-GFP plasmid (modified from Addgene #60958, by replacing the hSyn promoter with a CMV promoter) at the SapI restriction site for in vitro validation. .. Subsequently, the selected SpCas9 sgRNA (SpCas9 sgRNA4) was sub-cloned into a AAV package plasmid (pX552-hsyn-mCherry-YFP sgRNA2, sgRNA6 or pX552-LacZ sgRNA) at the MluI (catalog no. R3198; New England Biolabs) restriction site to generate YFP or LacZ targeting kamikaze CRISPR/Cas construct.

    Modification:

    Article Title: Utility of Self-Destructing CRISPR/Cas Constructs for Targeted Gene Editing in the Retina.
    Article Snippet: AgeI (catalog no. R0552S; New England Biolabs) digested pX551 plasmid was used as a positive control. .. Four SpCas9 sgRNAs were then cloned into a pX552‐CMV‐GFP plasmid (modified from Addgene #60958, by replacing the hSYN1 promoter with a CMV promoter) at the SapI restriction site for in vitro validation. .. Subsequently, the selected SpCas9 sgRNA (SpCas9 sgRNA4) was sub‐cloned into an AAV package plasmid (pX552‐hSYN1‐mCherry‐YFP sgRNA2, sgRNA6 or pX552‐LacZ sgRNA) at the MluI (catalog no. R3198; New England Biolabs) restriction site to generate YFP or LacZ targeting kamikaze CRISPR/Cas9 construct.

    Article Title: Efficacy and dynamics of self-targeting CRISPR/Cas constructs for gene editing in the retina
    Article Snippet: Agel (catalog no. R0552S; New England Biolabs) digested pX551 plasmid was used as a positive control. .. Four SpCas9 sgRNAs were then cloned into a pX552-CMV-GFP plasmid (modified from Addgene #60958, by replacing the hSyn promoter with a CMV promoter) at the SapI restriction site for in vitro validation. .. Subsequently, the selected SpCas9 sgRNA (SpCas9 sgRNA4) was sub-cloned into a AAV package plasmid (pX552-hsyn-mCherry-YFP sgRNA2, sgRNA6 or pX552-LacZ sgRNA) at the MluI (catalog no. R3198; New England Biolabs) restriction site to generate YFP or LacZ targeting kamikaze CRISPR/Cas construct.

    In Vitro:

    Article Title: Utility of Self-Destructing CRISPR/Cas Constructs for Targeted Gene Editing in the Retina.
    Article Snippet: AgeI (catalog no. R0552S; New England Biolabs) digested pX551 plasmid was used as a positive control. .. Four SpCas9 sgRNAs were then cloned into a pX552‐CMV‐GFP plasmid (modified from Addgene #60958, by replacing the hSYN1 promoter with a CMV promoter) at the SapI restriction site for in vitro validation. .. Subsequently, the selected SpCas9 sgRNA (SpCas9 sgRNA4) was sub‐cloned into an AAV package plasmid (pX552‐hSYN1‐mCherry‐YFP sgRNA2, sgRNA6 or pX552‐LacZ sgRNA) at the MluI (catalog no. R3198; New England Biolabs) restriction site to generate YFP or LacZ targeting kamikaze CRISPR/Cas9 construct.

    Article Title: Efficacy and dynamics of self-targeting CRISPR/Cas constructs for gene editing in the retina
    Article Snippet: Agel (catalog no. R0552S; New England Biolabs) digested pX551 plasmid was used as a positive control. .. Four SpCas9 sgRNAs were then cloned into a pX552-CMV-GFP plasmid (modified from Addgene #60958, by replacing the hSyn promoter with a CMV promoter) at the SapI restriction site for in vitro validation. .. Subsequently, the selected SpCas9 sgRNA (SpCas9 sgRNA4) was sub-cloned into a AAV package plasmid (pX552-hsyn-mCherry-YFP sgRNA2, sgRNA6 or pX552-LacZ sgRNA) at the MluI (catalog no. R3198; New England Biolabs) restriction site to generate YFP or LacZ targeting kamikaze CRISPR/Cas construct.

    Biomarker Discovery:

    Article Title: Utility of Self-Destructing CRISPR/Cas Constructs for Targeted Gene Editing in the Retina.
    Article Snippet: AgeI (catalog no. R0552S; New England Biolabs) digested pX551 plasmid was used as a positive control. .. Four SpCas9 sgRNAs were then cloned into a pX552‐CMV‐GFP plasmid (modified from Addgene #60958, by replacing the hSYN1 promoter with a CMV promoter) at the SapI restriction site for in vitro validation. .. Subsequently, the selected SpCas9 sgRNA (SpCas9 sgRNA4) was sub‐cloned into an AAV package plasmid (pX552‐hSYN1‐mCherry‐YFP sgRNA2, sgRNA6 or pX552‐LacZ sgRNA) at the MluI (catalog no. R3198; New England Biolabs) restriction site to generate YFP or LacZ targeting kamikaze CRISPR/Cas9 construct.

    Article Title: Efficacy and dynamics of self-targeting CRISPR/Cas constructs for gene editing in the retina
    Article Snippet: Agel (catalog no. R0552S; New England Biolabs) digested pX551 plasmid was used as a positive control. .. Four SpCas9 sgRNAs were then cloned into a pX552-CMV-GFP plasmid (modified from Addgene #60958, by replacing the hSyn promoter with a CMV promoter) at the SapI restriction site for in vitro validation. .. Subsequently, the selected SpCas9 sgRNA (SpCas9 sgRNA4) was sub-cloned into a AAV package plasmid (pX552-hsyn-mCherry-YFP sgRNA2, sgRNA6 or pX552-LacZ sgRNA) at the MluI (catalog no. R3198; New England Biolabs) restriction site to generate YFP or LacZ targeting kamikaze CRISPR/Cas construct.



    Similar Products

    95
    Addgene inc px552 u6 sp grna cmv gfp
    Px552 U6 Sp Grna Cmv Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px552+cmv+gfp+plasmid/PX552+(Plasmid+%2360958)/10__1134_slash_s0006297921070051-49-27-40
    Average 95 stars, based on 1 article reviews
    px552 u6 sp grna cmv gfp - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Addgene inc px552 cmv gfp plasmid
    Px552 Cmv Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px552+cmv+gfp+plasmid/PX552+(Plasmid+%2360958)/pm31373227-45-8-12
    Average 95 stars, based on 1 article reviews
    px552 cmv gfp plasmid - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    Image Search Results